Leucocytes and Thrombosis

Summary

The rate of white cell loss from heparinised whole blood in a rotating glass flask has been used to study leucocyte behaviour.

Leucocyte loss requires divalent cations and magnesium is more active than calcium. Sulphydryl blockers and potassium cyanide inhibit white cell loss, showing that sulphydryl groups and a glycolytic pathway play a part in leucocyte adhesion.

White cell loss from plasma rotated in a flask is negligible, but is markedly increased by adding red cells, red cell sonicates or ADP.

The role of platelets and of substances which affect platelet behaviour in white cell adhesion is discussed.

• References

• 1 Aledort L. M, Troup S. B, and Weed R. I. 1968; Inhibition of sulphydryl-dependent platelet functions by penetrating and non-penetrating analogues of parachloromercuribenzene. Blood 31: 471.
• 2 Banks D. C, and Mitchell J. R. A. 1973; Leucocytes and thrombosis. I. A simple test of leucocyte behaviour. Thrombosis et Diathesis Haemorrhagica 30: 36.
• 3 Bishop C, Rankine D. M, and Talbott J. H. 1959; The nucleotides in normal human blood. Journal of Biological Chemistry 234: 1233.
• 4 Böyum A. 1968 Separation of leucocytes from blood and bone marrow. Scandinavian Journal of Clinical Investigation. 21, Suppl. 97.
• 5 Evans C. A, and Middleton V. L. 1970; Evaluation of methods for preparing pure lymphocyte suspensions from peripheral blood. Journal of Clinical Pathology 23: 822.
• 6 Fisher T. N, and Ginsberg H. S. 1956; The reaction of influenza viruses with guinea pig polymorphonuclear leucocytes. 11. The reduction of white blood cell glycolysis by influenza viruses and receptor-destroying enzyme (RDE). Virology 2: 637.
• 7 Friedberg N. M, and Zucker M. B. 1971 ADP as the cause of reversible inhibition of platelet retention. Information exchange, 71-44.
• 8 Gaarder A, Jonsen J, Laland S, Hellem A, and Owren P. A. 1961; Adenosine diphosphate in red cells as a factor in the adhesiveness of human blood platelets. Nature 192: 531.
• 9 Garvin J. E. 1961; Factors affecting the adhesiveness of human leucocytes and platelets in vitro. Journal of Experimental Medicine 114: 51.
• 10 Hampton J. R, and Mitchell J. R. A. 1966; Effect of glass contact on the electrophoretic mobility of human blood platelets. Nature 209: 470.
• 11 Harrison M. J. G, Emmons P. R, and Mitchell J. R. A. 1966; The effect of sulphydryl and enzyme inhibitors on platelet aggregation in vitro. Thrombosis et Diathesis Haemorrhagica 16: 122.
• 12 Harrison M. J. G, and Mitchell J. R. A. 1966; The influence of red blood cells on platelet adhesiveness. Lancet 2: 1163.
• 13 Haslam R. J. 1964; Role of adenosine diphosphate in the aggregation of human blood platelets by thrombin and by fatty acids. Nature 202: 765.
• 14 Hellem A. J. 1960 The adhesiveness of human blood platelets in vitro. Scandinavian Journal of Clinical Investigation. 12, Suppl. 51.
• 15 Kvarstein B. 1969; a Effects of proteins and inorganic ions on the adhesiveness of human leucocytes to glass beads. Scandinavian Journal of Clinical and Laboratory Investigation 24: 41.
• 16 Kvarstein B. 1969; b Effect of some metabolic inhibitors on the adhesiveness of human leucocytes to glass beads. Scandinavian Journal of Clinical and Laboratory Investigation 24: 35.
• 17 Mitchell J. R. A, and Schwartz C. J. 1965. Arterial disease. Blackwell Scientific; Oxford:
• 18 Mitchell J. R. A, and Sharp A. A. 1964; Platelet clumping in vitro. British Journal of Haematology 10: 78.
• 19 Robinson C. W, Mason R. G, and Wagner R. H. 1963; Effect of sulphydryl inhibitors on platelet agglutinability. Proceedings of the Society of Experimental Biology, New York 116: 857.
• 20 Tyrode M. V. 1909; The mode of action of some purgative salts. Archives internationales de pharmacodynamic et de therapie 19: 205.